LOCUS ANGUSTA4 ELONGATA1 ELONGATA2 ELONGATA3 ELONGATA4 HEMIVENATA INCURVATA1 INCURVATA2 INCURVATA3 INCURVATA4 INCURVATA5 INCURVATA6 INCURVATA8 INCURVATA9 INCURVATA15 ONDULATA3 RETICULATA ROTUNDA1 ROTUNDA2 RUGOSA1 RUGOSA2 SCABRA3 TRANSCURVATA1 ULTRACURVATA1 ULTRACURVATA2 VENOSA1 VENOSA3 VENOSA4 VENOSA5 VENOSA6  
Gene name (first reference) ANG4 (Berná et al., 1999) ELO1 (Berná et al., 1999) ELO2 (Berná et al., 1999) ELO3 (Berná et al., 1999) ELO4 (Berná et al., 1999) HVE(Alonso-Peral et al., 2006) ICU1 (Serrano-Cartagena et al., 2000) ICU2 (Barrero et al., 2007) ICU3 (Serrano-Cartagena et al., 1999) ICU4 (Serrano-Cartagena et al., 1999) ICU5 (Serrano-Cartagena et al., 2000) ICU6 (Berná et al., 1999) ICU8 (Berná et al., 1999) ICU9 (Berná et al., 1999) ICU15 (Serrano-Cartagena et al., 1999) OND3 (Berná et al., 1999) RE (Redei, 1964) RON1 (Berná et al., 1999) RON2 (Cnops et al., 2004)     SCA3 (Hricová et al., 2006) TCU1 (Berná et al., 1999) UCU1 (Berná et al., 1999) UCU2 (Pérez Pérez et al., 2004) VEN1 (Berná et al., 1999)   VEN4 VEN5 (Berná et al., 1999)    
Alternative names (first reference) HUB1 (Fleury et al., 2006;  Liu et al., 2006)   ABO1 (Chen et al., 2006) HAG3 DRL1 (Nelissen et al., 2003) CAND1 (Cheng et al., 2004); Chuang et al., 2004; Feng et al., 2004) CLF (Goodrich et al., 1997)    HST (Telfer and Poethig, 1998) ATHB15 (Prigge et al., 2005) SHY2 (Kim et al., 1996) AXR3 (Leyser et al., 1996) HYL1(Lu and Fedoroff, 2000) AGO1(Bohmert et al., 1998) HEN1(Chen et al., 2002) ARC6 (Vitha  et al., 2003) VEN2 (Berná et al., 1999) SAL1 (Quintero et al., 1996) LUG (Conner and Liu, 2000)     RpoTp (Hedkte et al., 1997) AP22.15 BIN2 (Li et al., 2001) TWD1 (Kamphausen et al., 2002) trp5 (Li and Last, 1996)     RER3 (Pérez-Pérez et al., unpublished)    
  RDO4 (Liu et al., 2006)                 CORONA (Green et al., 2005) IAA3 IAA17         LCD1 (Barth and Conklin, 2003) HOS2 (Lee et al., 1999)         AP22_15 DWF12 (Choe et al., 2002) AtFKBP42 (Kamphausen et al., 2002) amt (Kreps and Town, 1992)        
                                                    tir7 (Ruegger et al., 1997)        
                                                    wei2 (Stepanova et al., 2005)          
Allele (reference) ang4-1  (hub1-1) elo1-1 (Berná et al., 1999) elo2-1 (Berná et al., 1999) elo3-1 (Berná et al., 1999) elo4-1 (Berná et al., 1999); drl1-4 (Nelissen et al., 2003) hve-1(Alonso-Peral et al., 2006)   icu2-1 icu3-1 (hst-5) (Telfer and Poethig, 1998) icu4-1 (Serrano-Cartagena et al., 1999) icu5 icu6 icu8-1 (hyl1-11) (Berná et al., 1999) icu9-1 (ago1-51) (Berná et al., 1999) hen1-2 (Serrano-Cartagena et al., 1999) ond3 (Berná et al., 1999) re-3 ron1-1 (Berná et al., 1999) ron2-1 rug1 (Berná et al., 1999) rug2-1 (Berná et al., 1999) sca3-1 (Berná et al., 1999) tcu1-1 (Berná et al., 1999) ucu1-1 (Berná et al., 1999) ucu2-1  ven1-1   ven4-1 ven5-1    
   Genetic background Ler Ler Ler Ler Ler Ei-5 (N1128)   En-2 En-2 En-2 En-2 Ler Ler Ler Ler Ler Ler Ler Ler Ler Ler Ler Ler Ler Ler Ler   Ler Ler  
   Origin EMS EMS EMS EMS EMS Spontaneous   AIS collection (unknown mutagen) Unknown Unkown AIS collection (unknown mutagen) EMS EMS EMS EMS EMS EMS EMS EMS EMS EMS EMS EMS EMS Fast neutrons EMS   EMS EMS  
   Mode of inheritance Recessive Recessive Recessive Recessive Recessive Recessive   Recessive recesive semidominant Dominant Semidominant recesive recesive recesive Recessive Recessive Recessive Recessive Recessive Recessive Recessive Recessive Semidominant Recessive Recessive   Recessive Recessive  
   Number of backcrosses 2 2 2 2 2                     2 2 2   5 4 2 2 2 2 2   3    
   Nature of the mutant allele Loss-of-function Loss-of-function Loss-of-function Loss-of-function Loss-of-function Loss of function   Loss-of-function   Gain-of-function Gain-of-function Gain-of-function hypomorphic hypomorphic hypomorphic Loss-of-function Loss-of-function Loss-of-function Loss-of-function Loss-of-function or hypomorphic Loss-of-function or hypomorphic Loss-of-function or hypomorphic Loss-of-function Gain-of-function or hypermorphic Loss-of-function            
Allele (reference) hub1-3 elo1-2 (Nelissen et al., 2005) elo2-2 (Nelissen et al., 2005) elo3-2 (Nelissen et al., 2005)   hve-2(Alonso-Peral et al., 2006)   icu2-2 icu3-2 (hst-21) (Berná et al., 1999) icu4-2 (Serrano-Cartagena et al., 1999)     icu8-2 (hyl1-12) icu9-2(ago1-52) (Berná et al., 1999) icu15-1(hen1-11) (Serrano-Cartagena et al., 1999) arc6 -1  (Pyke et al., 1994) re-4 ron1-2 (Robles et al.) ron2-2   rug2-2  sca3-2 (Hricová et al., 2006) tcu1-2 ucu1-2 (Berná et al., 1999) ucu2-2 ven1-2   ven4-2 ven5-2    
   Genetic background Col-0 Col-0 Col-0 Ws-2   Col-0   Col-0 Ler En-2     Ler Ler En-2 Ws-2 Ler Col-0 Ler   Col-0 Col-0 Col-0 Ler Col-o Col-0   Col-0 Ler  
   Origin T-DNA T-DNA T-DNA T-DNA   T-DNA   T-DNA EMS Unkown     Fast neutrons EMS Unknown   EMS T-DNA EMS   T-DNA T-DNA T-DNA EMS X-ray mutagenesis T-DNA   T-DNA EMS  
   Mode of inheritance Recessive Recessive Recessive Recessive   Recessive   Recessive recesive semidominant     recesive recesive recesive   Recessive Recessive Recessive   Recessive Recessive Recessive Semidominant Recessive Recessive   Recessive Recessive  
   Number of backcrosses                                 2       1 1   2 2 1        
   Nature of the mutant allele Loss-of-function Loss-of-function (incomplete penetrance) Loss-of-function (incomplete penetrance) Loss-of-function   Null   Loss-of-function hypomorphic Gain-of-function     Loss-of-function hypomorphic     Loss-of-function Loss-of-function Loss-of-function   Loss-of-function or hypomorphic Loss-of-function or hypomorphic Loss-of-function Gain-of-function or hypermorphic Loss-of-function            
Allele (reference) hub1-4 elo1-3 (Nelissen et al., 2005) elo2-3 (Nelissen et al., 2005) elo3-3 (Nelissen et al., 2005)   hve-3(Alonso-Peral et al., 2006)   icu2-3 icu3-3 (hst-22)           icu15-2 (hen1-12) (Serrano-Cartagena et al., 1999)   re-5         sca3-3 (Hricová et al., 2006) tcu1-3 ucu1-3 (Berná et al., 1999) ucu2-3     ven4-3 ven5-3    
   Genetic background Col-0 Col-0 Col-0 Ws-2   Col-0   Ler Col           En-2   Ws-2         Col-0 Col-0 Ler Col-0     Col-0 Ler  
   Origin T-DNA T-DNA T-DNA RNAi   T-DNA   Ds insertion ADN-T           Unknown   T-DNA         T-DNA T-DNA EMS T-DNA     T-DNA EMS  
   Mode of inheritance Recessive Recessive Recessive Recessive   Recessive   Recessive recesive           recesive   Recessive         Recessive Recessive Semidominant Recessive     Recessive Recessive  
   Number of backcrosses                                           1   2 2          
   Nature of the mutant allele Loss-of-function Loss-of-function (incomplete penetrance) Loss-of-function (incomplete penetrance) Loss-of-function   Null   Loss-of-function Loss-of-function               Loss-of-function         Loss-of-function or hypomorphic Loss-of-function Gain-of-function or hypermorphic Loss-of-function          
Scientists in charge   Pedro Robles Pedro Robles Pedro Robles Pedro Robles Pedro Robles       María Rosa Ponce María Rosa Ponce José Manuel Pérez José Manuel Pérez María Rosa Ponce María Rosa Ponce María Rosa Ponce José Manuel Pérez José Manuel Pérez Pedro Robles   Víctor Quesada Víctor Quesada Víctor Quesada María Rosa Ponce José Manuel Pérez José Manuel Pérez Pedro Robles   Víctor Quesada José Manuel Pérez    
                  Verónica Aguilera Verónica Aguilera     Verónica Aguilera Verónica Aguilera Verónica Aguilera   Rebeca González     Raquel Sarmiento Raquel Sarmiento   Almudena Ferrández     Almudena Mollá   Raquel Sarmiento Rebeca González  
                                                             
                                                             
MOLECULAR CLONING                                                              
Chromosome 2 3 5 5 1     5 3 1 1 1 1 1     2 5 4 5 4 2 4 4 3 5   5 3  
Linked markers, distance (cM)                                                          
Candidate BAC interval                                                          
Candidate genes                                                          
Entry At2g44950 At3g11220 At5g13680 At5g50320 At1g13870     At5g67100 At3g05040 At1g52150 At1g04240 At1g04250 At1g09700 At1g48410     At2g37860 At5g63980 At4g32551 At5g08280 At4g02990 At2g24120 At4g37130 At4g39400 At3g21640 At5g05730   At5g40270 At3g08640    
References for the cloning Fleury et al., 2006 Nelissen et al., 2005 Nelissen et al., 2005 Nelissen et al., 2005 Nelissen et al., 2003     Barrero et al., 2007 Bollman et al., 2003 Ochando et al., 2006 Pérez-Pérez et al., submitted Pérez-Pérez et al., submitted         González-Bayón et al., 2006 Quintero et al., 1996 Cnops et al., 2004     Hricová et al., 2006 Alonso-Peral et al., 2005 Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004 Niyogi and Fink, 1992     Pérez-Pérez et al, unpublished    
  Liu et al., 2006                               Barth and Conklin, 2003 Xiong et al., 2001 Conner and Liu, 2000       Li and Nam 2002 Kamphausen et al., 2002 Li and Last, 1996        
                                                Choe et al., 2002   Kreps et al., 1996          
Other references                     SHY2 arabidopsis: 11 references in Pubmed AXR3 arabidopsis: 24 references in Pubmed                   RpoTp arabidopsis: 7 references in Pubmed   BIN2 arabidopsis: 20 references in Pubmed TWD1 arabidopsis: 8 references in Pubmed Rutherford et al., 1998; Ruegger et al., 1997; Stepanova et al., 2005          
PROTEIN ENCODED                                                              
Size 878 aa (99.720 Kda) 355 aa (38.732 Kda) 1319 aa (146.627 Kda) 565 aa (63.695 Kda) 302 aa (34.064 Kda)     1492 aa,  1,202 aa, 133130.0 Kda               432 aa (46.6 Kda) 407aa (43.510 Kda) 931 aa 102Kda 382 aa, 41.04 kD 541 aa, 61.6 kD 993 aa, 112.6 kD 513 aa, 56,5 kda  XXX aa, XX Kda 365 aa, 42 Kda 595 aa, 66 Kda   473aa    
Putative domains RING finger domain       ATP/GTP binding domain     Polymerase catalitic domains I to VII ( Wang et al., 1989)    HOX, bZIP, START lipid-binding domain       PAZ and PIWI domain     Two transmembrane regions 3(2),5-bisphosphate nucleotidase SSDP (Single-stranded DNA-binding protein) Porphobilinogen deaminase Mitochodrial transcription termination factor-related RNA polymerase domain, etc. NLS Ser/Thr kinase domain, etc. FKBP-like domain     HD-domain    
                Domains A to E (Miyazawa et al., 1993)                   Inositol monophosphatase LisH motif         3 tetratricopeptide repeats (TPRs)           
Activity H2B monoubiquitinating E3 ligase Component of the Elongator complex Component of the Elongator complex Component of the Elongator complex Chromatin associated protein KTi12 which interacts with the Elongator complex     MIR domain   Transcription factor,regulates polarity specification of adaxial/abaxial axis, pattern of vasculature, function of meristems     biogenesis of microRNAs endonuclease activity, posttranscriptional regulatión by microRNAs     Unknown   Seven  WD40 repeats hydroxymethylbilane synthesis mitochondrial genome transcription (putative) RNA synthesis Nucleoporin (Alonso-Peral et al., 2005) Protein kinase of BZR1 (reference) FK506-binding protein (FKBP) Anthranilate synthase   phosphohydrolase    
        Histone Acetyl Transferase       E2F binding site motif TTTCCCGC of cell cycle-regulated genes                     Gln-rich domain         PPIase activity           
Function  General regulator of cell divisions Stimulation of cell proliferation Stimulation of cell proliferation Stimulation of cell proliferation Regulation of transcription elongation       nucleocytoplasmic transporter activity, negative regulation of flower development, polarity specification of adaxial/abaxial axis, leaf morphogenesis, root development, shoot development, pre-microRNA export from nucleus                Unknown Involved in the response to cold, drought and ABA   Chlorophyll biosynthesis   Chloroplast genome transcription Nucleocitoplasmatic transport (Alonso-Peral et al., 2005) Negative regulation of BR response (reference) Crosstalk auxin and BR signalling (Pérez-Pérez et al., 2004)          
Subcellular localization                                 Chloroplast     Chloroplastic (Lim et al., 1994) Mitochondria (putative) Chloroplastic (Hedtke et al., 1997, 1999) Nucleopore complex (Alonso-Peral et al., 2005) Cytoplasm (putative) Plasma membrane and tonoplast (Kamphausen et al., 2002) Chloroplast        
Others         Contains calmodulin-binding site                         Rescues sulfur assimilation mutants in yeasts         Interacts physically with XXX (reference) Interacts physically with AtMRP1 (Geisler et al., 2004)          
                putative catalytic subunit of DNA polymerase α                     Negative regulation of transcription in vegetative plant development (AGAMOUS repressor)                    
                Nuclear                     Nuclear                    
GENE CONSTRUCTS               Interacts physically with TFL2 in Vitro (Barrero et al., 2007)                                              
Promoter:GUS         Nelissen et al., 2003                       YES (Pérez-Pérez et al, unpublished) Xiong et al., 2001   NO NO NO YES (Alonso and Ferrández et al., 2005) NO NO YES (Bender and Fink, 1998)        
Promoter:GFP                                       NO NO NO NO NO NO NO        
35S:GENE Fleury et al., 2006 Nelissen et al., 2005   Nelissen et al., 2005                         YES (Pérez-Pérez et al, unpublished)     NO NO NO YES (Alonso and Ferrández et al., 2005) NO YES (Pérez Pérez et al., 2004) NO        
35S:GENE:GFP               NO                     NO NO NO NO YES (Alonso and Ferrández et al., 2005) NO NO NO        
Promoter:GENE               NO                     YES (Conner and Liu, 2000) NO NO NO NO NO NO NO        
Others               NO                     NO NO NO NO NO NO NO NO        
GENE EXPRESSION               NO                     NO                        
Genevestigator (alternative program) data cDNA-AFLP, Nelissen et al., 2005 cDNA-AFLP, Nelissen et al., 2005 cDNA-AFLP, Nelissen et al., 2005 cDNA-AFLP, Nelissen et al., 2005 cDNA-AFLP, Nelissen et al., 2005     NO                     NO                    
Microarray results Fleury et al., 2006   Nelissen et al., 2005 Nelissen et al., 2005 Nelissen et al., 2005                           NO                    
MORPHOMETRY-PHENOTYPE PARAMETERS                                                              
Fresh weigth plant Fleury et al., 2006             High expression in shoot apex. Low expression in leaves and stems                     All tissues tested including roots, shoots, stems, cauline leaves, and inflorescences ( Conner and Liu, 2000) Unpublished Unpublished   Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004          
Dry weigth plant Fleury et al., 2006                                     Unpublished Unpublished   Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004          
Rosette area                                       Unpublished Unpublished Ferrández-Ayela et al., in progess              
Rosette perimeter                                       Unpublished Unpublished Ferrández-Ayela et al., in progess              
Rosette compactness                                       Unpublished Unpublished Ferrández-Ayela et al., in progess              
Root length Fleury et al., 2006 Nelissen et al., 2005 Nelissen et al., 2005 Nelissen et al., 2005 Nelissen et al., 2005                             Unpublished Unpublished Hricová et al., 2006 Alonso-Peral et al., 2005 Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004          
Hypocotyl length                                       Unpublished Unpublished Alonso-Peral et al., 2005 Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004          
Leaf number                                       Unpublished Unpublished Alonso-Peral et al., 2005 Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004          
Average days to bolt                                       Unpublished Unpublished Ferrández-Ayela et al., 2007              
Primary stem length                                       Unpublished Unpublished   Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004          
Number of secondary stems               Barrero et al., 2007                       Unpublished Unpublished   Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004          
Silique length               Barrero et al., 2007                       Unpublished Unpublished   Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004          
Number of seeds per silique                                       Unpublished Unpublished   Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004          
Node leaf number under study First node leaves             Barrero et al., 2007                       Third leaves Third leaves Third leaves First and third leaves Third leaves Third leaves          
Petiole length Fleury et al., 2006                                         Alonso-Peral et al., 2005 Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004          
Lamina length Fleury et al., 2006                                           Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004          
Lamina width Fleury et al., 2006                                   All (leaf series)       Pérez-Pérez et al., 2002 Pérez-Pérez et al., 2004          
Lamina circularity Fleury et al., 2006                                   Cnops et al., 2004                    
Lamina area (mm2) Fleury et al., 2006             Barrero et al., 2007                     Cnops et al., 2004                    
Lamina perimeter (mm)               Barrero et al., 2007                     Cnops et al., 2004                    
   Adaxial epidermal cell area                Barrero et al., 2007                     Cnops et al., 2004     Ferrández-Ayela et al., in progess              
   Abaxial epidermal cell area               Barrero et al., 2007                     Cnops et al., 2004     Ferrández-Ayela et al., in progess              
   Adaxial epidermal cell perimeter                Barrero et al., 2007                           Ferrández-Ayela et al., in progess              
   Abaxial epidermal cell perimeter                                     Cnops et al., 2004     Ferrández-Ayela et al., in progess              
   Adaxial epidermal cell number/area unit                                           Ferrández-Ayela et al., in progess              
   Abaxial epidermal cell number/area unit                                           Ferrández-Ayela et al., in progess              
   Number of stomata/adaxial area unit                                           Ferrández-Ayela et al., in progess              
   Number of stomata/abaxial area unit                                     Cnops et al., 2004     Ferrández-Ayela et al., in progess              
   Mesophyll cell area                                           Ferrández-Ayela et al., in progess              
   Mesophyll cell perimeter                                           Ferrández-Ayela et al., in progess              
   Mesophyll cell number                                           Ferrández-Ayela et al., in progess              
   Venation length (mm)                                     Cnops et al., 2004     Ferrández-Ayela et al., in progess              
   Vascular density (mm/mm2)                                           Ferrández-Ayela et al., in progess              
   Branching points                                     Cnops et al., 2004     Ferrández-Ayela et al., in progess              
   Branching points/mm2                                           Ferrández-Ayela et al., in progess              
   Number of free ends                                           Ferrández-Ayela et al., in progess              
   Number of free ends/mm2                                           Ferrández-Ayela et al., in progess              
   Trichome density                                           Ferrández-Ayela et al., in progess              
HORMONE RESPONSES                                                              
Auxins                         (Lu and Fedoroff, 2000)                 More sensitive than WT More sensitive than WT            
Auxin transport inhibitor                         (Lu and Fedoroff, 2000)                     More sensitive than WT          
Cytokinins                         (Lu and Fedoroff, 2000)                                
Gibberellins                                           Same as WT Same as WT Same as WT          
Brassinosteroids                                           Same as WT Less sensitive than WT Less sensitive than WT          
ABA                         (Lu and Fedoroff, 2000)                                
Ethylene                                                          
Jasmonic acid                                                          
Others                                           More sensitive than WT to PAC (Gibberellin inhibitor)              
                                                           
                                                           
                                                           
RESPONSES TO ENVIRONMENT                                                              
High light                                       More sensitive than WT More sensitive than WT                
Low light                                                          
High sucrose                                                          
Low sucrose                                       More sensitive than WT More sensitive than WT More sensitive than WT                
High temperature                                                          
Low temperature                                           More sensitive than WT                
Dark growth                                          Same as WT Same as WT Same as WT Photomorphogenic Same as WT          
Others